ABSTRACT
<p><b>AIM</b>To study the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes.</p><p><b>METHODS</b>The neonatal rat cardiomyocytes were cultured. According to the different grow period of neonatal rat cardiomyocytes, the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes were studied.</p><p><b>RESULTS</b>The efficiency was significantly increased after pEGFP-N1 plasmid transfection in one-day cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus.</p><p><b>CONCLUSION</b>The efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes was related to the grow period of neonatal rat cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus.</p>